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一、课程基本情况 课程编号：01140490 课程总学时：32，其中：讲课：0，实验：32。 课程学分：2.0 开课单位：植物生产类实验教学中心 课程分类：必修 开设学期：春 适用专业：植物保护专业 《农药学》实验部分 Experiments of Pesticide Science 一、课程基本情况 课程总学时：18，其中：讲课：0，实验：18。 所需先修课：普通植物病理学、普通昆虫学、农药学。 课程负责人：刘鹏飞 史雪岩 梁沛 二、课程内容简介 “农药学”课程是植保学科本科生专业教育理论课必修课程，农药学课程相关实验的学习，可使学生理论与实践相结合，通过基本实验操作的学习和基本实验现象的分析更透彻的理解理论课教学内容，并掌握分析和解决实际科研问题的基本方法。内容主要包括：农药剂型加工，杀虫剂、杀菌剂和除草剂生物测定，农药田间药效试验等内容。课程设置中注重经典的农药学验证实验与设计性试验相结合，使学生在掌握基本实验技能的基础上，培养其通过科学实验设计和实施解决实际科研问题的能力，具有一定的深度。 "Pesticide Science" course is one of professional education courses for undergraduate. Pesticide experiments course will enable students to combine theory and practice through the operation and analysis of the basic experiments. The course content mainly includes pesticide formulations preparation, insecticides, fungicides and herbicides bioassay, pesticide field trials and so on. Curricula focus on the classic pesticide experiments and design test combined, in order to make students master basic skills, as well as develop their scientific study ability to solve practical problems. 三、各部分教学纲要 实验一 10％咪鲜胺乳油和微乳剂的加工技术（4学时） 实验类型：设计性试验 目的要求：了解乳油和微乳剂的性能要求、制剂组成和各组分的作用；乳油和微乳剂配制和质量检测方法；掌握乳油和微乳剂配制过程中乳化剂筛选的基本方法及农药冷热贮藏的实验方法。 内容包括：1)掌握乳油和微乳剂剂型特点、配制的基本方法； 2)了解乳油和微乳剂的质量检测方法； 3)通过比较实验明确微乳剂的环境相容性和加工难点。 实验二 杀菌剂生物测定（4学时） 实验类型：验证性实验 目的要求：了解杀菌剂生物测定技术的意义、内容、一般原理和原则；常规的杀菌剂生物测定方法。 内容包括：1)掌握杀菌剂生物测定的常规方法：生长速率测定法； 2)学习毒力曲线、回归方程和EC50值的求得方法。 实验三 杀虫剂生物测定－药膜法触杀毒力测定（3学时） 实验类型：验证性实验 目的要求：了解杀虫剂生物测定技术的意义、内容、一般原理和原则；常规的杀虫剂生物测定方法。 内容包括：1)杀虫剂初步毒力测定和精密毒力测定的原理和方法； 2)观察试虫中毒征象特性——兴奋、击倒、抽搐、麻痹、昏迷、呕吐、变色复活、死亡等，判断药剂是否需要继续进行精密毒力测定； 3）掌握药膜法精确测定药剂的触杀毒力，学习毒力曲线、回归方程和LD50值的求得方法。 实验四 除草剂的生物测定－高粱根茎法（3学时） 实验类型：验证性实验 目的要求：掌握除草剂生物测定的实验一般原理和常规方法，学习利用高粱根茎法测定除草剂毒力的实验方法。 内容包括：1)非光合作用抑制剂类除草剂的筛选和活性测定的高粱根茎法的实验原理、方法；2)掌握应用microsoft excel软件求算IC50值的方法。 实验五 杀虫剂和杀菌剂的田间药效试验（4学时） 实验类型：设计性试验 目的要求：了解农药田间药效试验设计、小区安排、试验条件安排等；掌握施药器械选择与校准方法；学习试验结果调查、记录和测量方法。 内容包括：1）杀菌剂田间喷雾的实施； 2）杀虫剂的熏烟施药方法； 3）杀虫剂和杀菌剂的田间药效试验设计和结果调查。 四、使用教材 《农药学实验指导》，刘西莉，刘鹏飞，2006年10月。 执笔人：刘鹏飞 审定人： 《植物检疫学》实验教学大纲 Experiments of Plant Quarantine 一、课程基本情况 课程总学时：14，其中：讲课：0，实验：14，上机：0，实习：0，课外：0。 所需先修课：普通昆虫学、普通植物病理学、农药与植物检疫学 课程负责人：王登 刘星月 二、课程内容简介 植物检疫学实验是植物保护专业学生的一门必修课。本课程与植物检疫学课程相辅相承，通过检疫性病虫草实物标本及部分种类的影像图片的观察、比较、鉴别，使学生能够对检疫性病虫杂草种类及其鉴别特征有初步的认识；通过具体有害生物适生性风险分析软件的使用，了解有害生物风险分析的基本方法。提高学生对于植物检疫性有害生物和有害生物风险分析方法的认知水平。 “Experiments of Pesticide and Plant Quarantine” is a required course facing to undergraduates majoring in plant protection. The experiments covered two parts: master the morphological characteristics of the important quarantine pests including insects, diseases, weeds; and know some methods of Pest Risk Analysis. The main aim is to improve practical knowledge about quarantine pests and basic methods of Pest Risk Analysis. 三、各部分教学纲要 实验一 重要检疫性病原物的识别与鉴定（4学时） 实验类型：演示/验证 内容提要：观察烟草霜霉病（Peronospora tabacina Adam），梨火疫病菌（Erwinia amylovora (burrill) Winslow et al.），番茄环斑病毒（Tobacco ring spot virus）和松材线虫（Bursaphelenchuh xylophilus (Steiner & Buhrer) Nickle）的危害标本和病原物标本，掌握真菌、细菌、病毒、类菌原体、线虫等检疫性病原物危害症状特征及一些病源菌的主要形态特征。 实验报告：绘制烟草霜霉病，梨火疫病菌，番茄环斑病毒和松材线虫的主要形态特征。 实验二 重要检疫性昆虫的识别与鉴定（4学时） 实验类型：演示/验证 内容提要：观察橘小实蝇（Bactrocera dorsalis Hendel），菜豆象（Acanthoscelides obtectus Say），苹果蠹蛾（Cydia pomonella L），高粱瘿蚊（Contarinia sorghicola Coquillett）的标本，掌握其主要鉴定特征，了解双翅目、鞘翅目、鳞翅目和其他类检疫性昆虫的识别特征。 实验报告：绘制橘小实蝇、菜豆象、苹果蠹蛾和高粱瘿蚊的主要形态特征并注明名称。 实验三 重要检疫性杂草的识别与鉴定（3学时） 实验类型：演示/验证 内容提要：以五角菟丝子(Cuscuta pentagona Engelm.)，毒麦(Lolium temulentum L.)和假高粱（Sorghum halepense L. Pers）为标本，识别寄生型、有毒型和竞争型检疫性杂草。 实验报告：绘制五角菟丝子，毒麦和假高粱的外形，标出各器官的名称。 实验四 有害生物适生性风险分析软件的使用（3个学时） 实验类型：演示/验证 内容提要：以使用CLIMEX软件对墨西哥按实蝇（Anastrepha ludens Loew）在我国的适生范围与适生程度分析为例，演示有害生物适生性分析软件在使用过程中的参数指标体系设定，以及适生性结果的评判。 实验报告：列举常用有害生物适生性风险分析软件及CLIMEX使用中涉及的参数（墨西哥按实蝇）及结果评价。 四、使用教材及主要参考书。 使用教材： 许志刚主编，植物检疫学，北京：高等教育出版社，2008 主要参考书： 1.陈乃中，中国进境检疫性有害生物，北京：中国农业出版社，2009 2.万方浩等，生物入侵：技术篇，北京：中国科技出版社，2008 3.全国农业技术推广中心．植物检疫性有害生物图鉴．北京：中国农业出版社，2001. 执笔人：王登 审定人： Experiments of Plant Protection 3 1. Basic information Course code: 01140490 Total teaching hours: 32 hours, among which 18 hours for Experiments of Pesticide Science, 14 hours for Experiments of Plant Quarantine Credits: 1.0 Type of the course: compulsory Teaching terms: spring Owner of the course: Experimental teaching center for plant production Majors applicable: Plant Protection Prerequisites: General Entomology, General Plant Pathology, Pesticide Science and Plant Quarantine Person in charge of the course: Experiments of Pesticide Science: Liu Pengfei, Shi Xueyan and Liang Pei Experiments of Plant Quarantine: Wang Deng and Liu Xingyue Experiments of Pesticide Science 2. Course description This course is designed for a good understanding of the concepts, terminology, and theory of pesticide science through practical experimental training of undergraduate students. Special emphasis is placed on design and preparation of pesticide formulation, bioactivity determination of insecticides, fungicides and herbicides, and the application of pesticides in crop disease and insect management. Finally, the calibration of equipment of sprayer will be introduced in field trial part. The objective of this assignment is to ensure the knowledge of pesticides and their application be readily available and easily understood by students. 3. Design of each teaching section Experiment 1: Preparation of 10% Prochloraz EC and ME (4 teaching hours) Type of experiment: designing Brief contents: 1) Preparing 10% Prochloraz EC and ME with offered pesticide adjuvants. 2) Detecting the qualities of 10% Prochloraz EC and ME. 3) Learning the difference of EC and ME in environmental compatibility and main points in their preparation. Targets: Be able to correctly choose appropriate pesticide adjuvants and dosages for preparation of EC and ME formulation. Know the properties of basic formulation components and the method to detect the quality of EC and ME. Experiment 2: Bioactivity determination of fungicides-growth rate method (4 teaching hours) Type of experiment: presentation/verification Brief contents: 1) Testing the bioactivity of a fungicide by growth rate method. 2) Leaning the method to get a toxicity regression curve, toxicity regression equation and EC50. Targets: Learning the significance, principle, and basic methods of bioactivity determination of fungicides. Experiment 3: Bioactivity determination of insecticides-contact toxicity method (3 teaching hours) Type of experiment: presentation/verification Brief contents: 1) Testing the bioactivity of an insecticide by contact toxicity method 2) Observing insecticides poisoning symptoms including excitement, knocking down, convulsions, paralysis, pass out, vomiting, revival and death ect. 3) Leaning the method to get a toxicity regression curve, toxicity regression equation and LD50. Target: Realizing the significance and principle, and furthermore, mastering the basic methods of bioactivity determination of insecticides. Experiment 4: Bioactivity determination of herbicides-sorghum indicating method (3 teaching hours) Type of experiment: presentation/verification Brief contents: 1) Testing the bioactivity of a herbicide by sorghum indicating method. 2) Leaning the method to get a toxicity regression curve, toxicity regression equation and LD50. Targets: Realizing the significance and principle, and furthermore, mastering the basic methods for bioactivity determination of herbicides. Experiment 5: Field Trial of insecticides and fungicides (4 teaching hours) Type of experiment: designing Brief contents: 1) The spray of fungicide in the field trial and the calibration of equipment. 2) The application of insecticide by smoke method. 3) Designing, implementation and result recording of pesticides field trial. Targets: Learning the significance and basic principle of field trial of pesticides. Master the method of instrument calibration and selecting. 4. Teaching materials or references: Liu Xili and Liu Pengfei. 2006, Experiments guide of pesticide science，China Agricultural University Press (Beijing) Written by: Liu Pengfei, Shi Xueyan and Liang Pei Authorized by: Composed on April, 2011 Experiments of Plant Quarantine 2. Course description “Experiments of Pesticide and Plant Quarantine” is a required course facing to undergraduates majoring in plant protection. The experiments covered two parts: master the morphological characteristics of important crop pests including insects, diseases, weeds; and visit a place of treatment technology for plant quarantine. The main aim is to improve practical knowledge about quarantine pests and some methods of Pest Risk Analysis. 3. Design of each teaching section Experiment 1: Recognition and identification of important quarantine plant pathogens (4 class hours) Type of experiment: presentation/verification Brief contents: Observing the specimens of the blue mold of tobacco（Peronospora tabacina Adam）, the fireblight（Erwinia amylovora (Burrill) Winslow et al.）, the Tomato ring spot virus (TRSV), and the pinewood nematode（Bursaphelenchuh xylophilus (Steiner & Buhrer) Nickle）. Learning the major diagnosis and morphological characters of some quarantine plant pathogens (fungi, bacteria, virus, phytoplasma, and nematode). Experiment report: Sketching the major morphological characters of the blue mold of tobacco, fireblight, TRSV, and pinewood nematode. Experiment 2: Recognition and identification of important quarantine insects (4 class hours) Type of experiment: presentation/verification Brief contents: Observing the specimens of the Colorado potato beetle（Leptinotarsa decemlineata (Say) ）, the fall webworm（Hyphantria cunea (Drury) ）, the Oriental fruitfly（Bactrocera dorsalis Hendel）, the sorghum midge（Contarinia sorghicola Coquillett）. Learning the diagnostic characters and the identification approaches of the major quarantine insects in the orders Coleoptera, Lepidoptera, Diptera etc. Experiment report: Sketching the major morphological characters of the fall webworm, the Oriental fruitfly, and the sorghum midge, and marking their terms. Experiment 3: Recognition and identification of important quarantine weed (3 class hours) Type of experiment: presentation/verification Brief contents: Observing the specimens of ficld dldder(Cuscuta pentagona Engelm.), poison ryegrass (Lolium temulentum L.) and Johnsongrass（Sorghum halepense L. Pers）. Identified parasitic, poisonous and competing quarantine weed species. Experiment report: Sketching the major morphological characteristics of ficld dldder, poison ryegrass and Johnsongrass, and marking the organic terms. Experiment 4. Visit Supervisory and Auditing Office, Capital International Airport Quarantine Department, Beijing Entry-exit Inspection and Quarantine Bureau. (3 class hours) Type of experiment: presentation/visiting Brief contents: Visit Supervisory and Auditing Office, Capital International Airport Quarantine Department, Beijing Entry-exit Inspection and Quarantine Bureau. Learning the settings of relevant quarantine organization, the process of quarantine, and the quarantine methods by the presentations of staff and visit on-site. Experiment report: Describing the settings of Beijing quarantine organization. Give an example of process about a quarantine insects or plant pathogens. 4. Teaching materials or references Li Zhihong et al. 2008General Animal and Plant Quarantine，China Agricultural University Press (Beijing) Chen Naizhong. 2009China Entry Quarantine Pest，China Agriculture Press (Beijing) Wan Fanghao et al. 2008 Biology Inbreak：Technology，China Science and Technology Press (Beijing) National Agro-tech Extension and Service Center. 2001 Illustrated book of Plant Quarantine Pest．China Agriculture Press (Beijing) Written by: Wang Deng and Liu Xinyue Authorized by:
Composed on April, 2011
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