一、课程基本情况

课程编号：01140500

课程总学时：16，其中：实验：16。

课程学分：0.5

课程分类：必修

开课学期：秋

开课单位：植物生产类实验教学中心

适用专业：种子科学与工程

所需先修课：种子生物学

课程负责人：孙群 董学会

二、课程内容简介

种子生物学是研究植物种子的特征特性和生命活动规律及加工贮藏理论的一门应用学科,是种子科学与工程专业的主要专业课程之一。种子科学实验（一）是配合种子生物学的实验课程，也是种子科学与工程专业的必修课之一。本课程通过7个关键的种子生物学实验，使学生掌握种子生物学的基本实验技术和研究方法。

三、各部分教学纲要

实验1 种子形态结构鉴定

实验类型：专业实验

学时：2学时

基本内容：本实验要求学生通过对主要作物种子外部形态、内部结构的观察，了解各种子的主要特性，并运用这些特性进行种子鉴别。

实验室开放情况：否

实验2 种子平衡水分的测定

实验类型：专业实验

学时：2学时

基本内容：本实验要求学生通过练习测定种子平衡水分的测定方法，进一步了解种子在不同贮藏条件下吸湿变化的规律，以便及时做好仓库种子的管理工作。

实验室开放情况：否

实验3 种子中ATP 含量的测定

实验类型：专业实验

学时：2学时

基本内容：要求学生了解ATP测定原理，掌握荧光发测定ATP含量的方法和步骤。

实验室开放情况：否

实验4 种子老化试验

实验类型：专业实验

学时：2学时

基本内容：要求学生了解种子老化原理和注意事项，掌握种子老化方法和操作步骤。

实验室开放情况：否

实验5 种子活力测定

实验类型：专业实验

学时：3学时

基本内容：要求学生了解种子活力测定常用方法，掌握低温发芽测定棉花种子活性。

实验室开放情况：否

实验6 种子电导率的测定

实验类型：专业实验

学时：2学时

基本内容：本实验要求学生掌握电导率测定原理、电导率仪使用方法及种子电导率测定标准方法。

实验室开放情况：否

实验7 种子内源激素含量的测定

实验类型：专业实验

学时：3学时

基本内容：本实验要求学生掌握植物激素的ELISA测定原理、步骤和计算方法，以加深对植物激素在种子形成发育、休眠、衰老等过程中的调节作用的认识。

实验室开放情况：否

四、使用教材

尹燕枰，董学会. 种子学实验技术（第1版）. 北京：中国农业出版社，2008.

 

执笔人：孙群  董学会

审定人：王建华

                                              2011年5月10日修订

 

Experiments of Seed Science 1

1. Basic information

Course code: 01140500

Total teaching hours:   16  , among which  0  hours for lectures,  16  hours for experiments,  0  hours for on-line teaching. 

Credits: 0.5

Type of the course: compulsory

Teaching terms: autumn

Owner of the course: Experimental teaching center for plant production

Majors applicable: Seed Science and Technology

Prerequisites: Seed Biology

Person in charge of the course: Dr. Sun Qun and Dr. Dong Xuehui

2. Course description

Seed biology is the study on the characteristics and life process of plant seeds and the principle of seed storage and processing. It is one of the key courses for the major of seed science and engineering. Experiments of Seed Science 1 is offered in combination with key experiments of Seed biology. This course covers 7 key seed biology experiments for students mastering basic experimental techniques and research methods of seed biology.

3. Design of each teaching section

Experiment 1 Seed Morphology Identification

Experiment Type: Specialized Experiment

Teaching hours: 2

Basic components: This experiment requires students to understand the main characteristics of major crop seeds by observing external morphology, and use these features to identify seeds.

Laboratory opening: No

Experiment 2 Seed Equilibrium Moisture Content Determination

Experiment Type: Specialized Experiment

Teaching hours: 2

Basic components: This experiment requires students to possess the method of seed EMC determination, and have a further understanding the rules of seed moisture absorption changes under different storage conditions. It can helps to manage seeds in warehouse timely.

Laboratory opening: No

Experiment 3 Seed ATP Content Testing

Experiment Type: Specialized Experiment

Teaching hours: 2

Basic components: In this experiment, students are required to understand the principle of ATP fluorescence measurement, and master the methods and procedures.

Laboratory opening: No

Experiment 4 Seed Aging Experiment

Experiment Type: Specialized Experiment

Teaching hours: 2

Basic components: Require students to know the principles and considerations in seed aging, master seed aging methods and operating procedures.

Laboratory opening: No

Experiment 5 Seed Vigor Test

Experiment Type: Specialized Experiment

Teaching hours: 3

Basic components: Require students to understand commonly used methods for seed vigor test, and determinate seed vigor in cotton by cold germination test.

Laboratory opening: No

Experiment 6 Seed Conductivity Measurement

Experiment Type: Specialized Experiment

Teaching hours: 2

Basic components: This experiment requires students to know the principle of conductivity measurement, the usage of conductivity meter and standard methods for seed conductivity determination.

Laboratory opening: No

Experiment 7 Seed Endogenous Hormone Content Determination

Experiment Type: Specialized Experiment

Teaching hours: 3

Basic components: This experiment requires students to master the principles, procedures and calculation methods of ELISA. In order to enhance understanding of the plant endogenous hormone play regulatory role in seed formation, development, dormancy and aging. 

Laboratory opening: No

4. Teaching materials or references:

Yin Yanping，Dong Xuehui. Experimental Technology of Seed Science（1st Edition）. Beijing：China Agriculture Press，2008

Written by Dr. Dong Xuehui and Dr. Sun Qun

Authorized by Prof. Wang Jianhua

                                              Composed on May 10, 2011