Systematic studies of Diterpenoid Natural Product Biosynthesis | |||||||
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题 目: Systematic studies of Diterpenoid Natural Product Biosynthesis 报告人:Reuben J. Peters, Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology Professor, Iowa State University 时 间:2017年4月19日(周三)下午3:00 地 点:中国农业大学西校区生命科学研究中心4057(第七会议室) 邀请人:李召虎教授
Research Focus: Prof. Peters group has taken a systematic approach to investigating biosynthesis of diterpenoid natural products, particularly those whose production resembles that of the important gibberellin phytohormones in their initiation by a sequential pair of cyclization reactions catalyzed by class II diterpene cyclases and class I diterpene synthases, respectively. This is almost invariably followed by further transformations mediated by cytochrome P450 mono-oxygenases. Also Prof. Peters group studies range from enzymatic mechanisms and engineering to elucidation of metabolic pathways and networks, on up to physiological and ecological roles of the resulting natural products. While much of their work is centered on the important cereal crop rice (Oryza sativa), Prof. Peters group also investigates other plants, including the important Chinese medicinal herb Salvia miltiorrhiza (Danshen), as well as microbes, both bacteria and fungi. Recent results from Prof. Peters group studies on diterpenoid biosynthesis in rice and maize (Zea mays), as well as bacterial production of gibberellins, will be presented.
Some Recent Publications: Nett, R.S., Montanares, M., Marcassa, A., Lu, X., Nagel, R., Charles, T., Hedden, P., Rojas, M.C., Peters, R.J. (2017)“Elucidation of gibberellin biosynthesis in bacteria reveals convergent evolution”, Nat. Chem. Biol., 13(1):69-74. Nagel, R., Turrini, P., Nett, R.S., Leach, J.E., Verdier, V., Van Sluys, M.-A., Peters, R.J.(2017) “An operon for production of bioactive gibberellin A4 phytohormone with wide distribution in the bacterial rice leaf streak pathogen Xanthomonas oryzae pv. oryzicola”, New Phytol., in press. Jia, M., Zhou, K., Tufts, S., Schulte, S., Peters, R.J. (2017) “A pair of residues that interactively affect diterpene synthase product outcome”, ACS Chem. Biol., in press. Kumar, S., Kempinski, C., Zhuang, X., Norris, A., Mafu, S., Zi, J., Bell, S., Nybo, S., Kinison, S., Jiang, Z., Goklany, S., Linscott, K., Chen, X., Jia, Q., Brown, S., Bowman, J. Babbitt, P. Peters, R.J., Chen, F., Chappell, J. (2016) “Molecular Diversity of Terpene Synthases in the Liverwort Marchantia polymorpha”, Plant Cell, 28(10):2632-2650. Kitaoka, N., Wu, Y., Wang, Q., Zi, J., Peters, R.J. (2016) “Investigating inducible shortchain alcohol dehydrogenases/reductases clarifies rice oryzalexin biosynthesis”, Plant J., 88(2):271-279. Fu, J., Ren, F., Lu, X., Mao, H., Xu, M., Degenhardt, J., Peters, R.J., Wang, Q. (2016) “A tandem array of ent-kaurene synthases in maize with roles in gibberellin and more specialized metabolism”, Plant Physiol., 170(2):742-751. Potter, K.C., Zi, J., Young, Y.J., Schulte, S., Malchow, B.K., Tantillo, D.J., Peters, R.J.(2016) “Blocking deprotonation with retention of aromaticity in a plant ent-copalyl diphosphate synthase leads to product rearrangement”, Angew. Chem. Int. Ed., 55(2):634-638. Mafu, S., Jia, M., Zi, J., Morrone, D., Wu, Y., Xu, M., Hillwig, M.L., Peters, R.J. (2016)“Probing the promiscuity of ent-kaurene oxidase via combinatorial biosynthesis”, Proc. Natl. Acad. Sci. U.S.A., 113(9):2526-2531. Potter, K.C., Jia, M., Young, Y.J., Tantillo, D.J., Peters, R.J. (2016) “Product rearrangement from altering a single residue in the rice syn-copalyl diphosphate synthase”, Org. Lett., 18(5):1060-1063. |
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